2.

The effect of LH3 siRNAs on HT-1080 cells. (A) Galactosyltransferase (GT) and glucosyltransferase (GGT) activities in untransfected (white), vector transfected (pRNA U6.1/Neo) (grey) and LH3 siRNA (black) expressing HT-1080 cells after 10 days and 20 days of the treatment. The untreated cells were taken as 100%. The data presented were the mean values from two or three assays. (B) Light microscopic view at 10 days after transfection of HT-1080 cells transfected either with empty pRNA U6.1/Neo vector (control) or with LH3 siRNA-expressing constructs. The vector transfected cells grew normally with no obvious morphological change, whereas the LH3 siRNA transfected cells showed growth retardation, an abnormal rounded shape (a sign of cell detachment), and finally cell death.