8.

Immunofluorescence staining of His-tag proteins in permeabilized HT-1080 cells overexpressing full-length LH3 in native or mutated forms. Human LH3 and mutant LH3s were overexpressed using a pcDNA3 expression vector. The cells were constantly under selection pressure with 750 μg/ml G418 for one month, then stained by His-tag antibody. (A) pcDNA3 control. (B) Full-length human LH3 transfected cells. (C) Glycosyltransferase-deficient LH3 transfected cells. (D) LH-deficient LH3 transfected cells. A change in cell morphology was observed when the glycosyltransferase-deficient LH3 was overexpressed in the cells (C). Some non-specific staining was seen, as in A with His-tag antibodies.