1.
Monoclonal and polyclonal antibodies used for flow cytometry, immunofluorescence stainings and Western blotting
| Antibody | Clone | Isotype | Dilution | Source | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CD14, PE-labelled | rmC5-3 | lgG1 | 1:5 | BD Pharmingen, San Diego, CA, USA | |||||||||
| CD31, PE-labelled | JC70A | lgG1 | 1:50 | Dako, Glostrup, Denmark | |||||||||
| WM-59 | lgGl | 1:5 | BD Pharmingen, San Diego, CA, USA | ||||||||||
| CD34, FITC-labelled | 581 | lgGl | 1:5 | BD Pharmingen, San Diego, CA, USA | |||||||||
| CD36, PE-labelled | SMO | lgM | 1:20 | Ancell Corp., Bayport, MN, USA | |||||||||
| CD54 (ICAM-1) FITC-labelled | HA58 | lgGl | 1:5 | BD Pharmingen, San Diego, CA, USA | |||||||||
| CD62E (E-selectin) FITC-labelled | 1.2B6 | lgGl | 1:25 | Dako, Glostrup, Denmark | |||||||||
| CD105 (endoglin) PE-labelled | 266 | lgGl | 1:20 | BD Pharmingen, San Diego, CA, USA | |||||||||
| CD133 FITC-labelled | AC133 | lgGl | 1:100 | Abcam Ltd., Cambridge, UK | |||||||||
| lgGl | 1:25 | Miltenyi Biotech, Bergisch Gladbach, Germany | |||||||||||
| CD144 (VE-cadherin) PE-labelled | 55-7H1 | lgGl | 1:25 | BD Pharmingen, San Diego, CA, USA | |||||||||
| β2-microglobulin PE-labelled | 246-E8.E7 | lgG2a | 1:25 | Neomarkers, Fremont, CA, USA | |||||||||
| HLA-I PE-labelled | G46-2.6 | lgGl | 1:5 | BD Pharmingen, San Diego, CA, USA | |||||||||
| HLA-II PE-labelled | G46-6 | lgG2a | 1:5 | BD Pharmingen, San Diego, CA, USA | |||||||||
| LYVE-1 | Polyclonal | Rabbit serum | 1:400 | DCS Hamburg, Germany | |||||||||
| Polyclonal | Rabbit serum | 1:100 | Fitzgerald Industries International Inc., Concord, MA, USA | ||||||||||
| Podoplanin | Polyclonal | lgG1 | 1:200 | Acris, Hiddenhausen, Germany | |||||||||
| gp36 | lgGl | 1:100 | Fitzgerald Industries International Inc., Concord, MA, USA | ||||||||||
| Prox-1 | Polyclonal | Rabbit serum | 1:200 | RELIA Tech, Braunschweig, Germany | |||||||||
| Ployclonal | Rabbit serum | 1:100 | Fitzgerald Industries International Inc., Concord, MA, USA | ||||||||||
| Tie-1 | Polyclonal | 1:200 | Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA | ||||||||||
| Tie-2 | Polyclonal | 1:200 | Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA | ||||||||||
| VEGFR-1/FU-1 | Polyclonal | 1:200 | Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA | ||||||||||
| VEGFR-2/Flk-1 | Monoclonal | 1:100 | Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA | ||||||||||
| VEGFR-3 | Monoclonal | 1:100 | Chemicon International, Temecula, CA, USA | ||||||||||
| Goat IgG | 1: 50 | RD Systems, Inc., Tustin, CA, USA | |||||||||||
| von Willebrand Factor (FVIII) | F8/86 | IgGl | 1:50 | Dako, Glostrup, Denmark | |||||||||
| Polyclonal | Rabbit serum | 1:100 | Dako, Glostrup, Denmark | ||||||||||