1.
Murine HL-1 cardiomyocytes express P2X1,3,4,5,6,7 and P2Y2,4,6,14. The presence of P2 receptors was assessed using ad hoc designed RT-PCR primers for all cloned murine P2 receptors. For each receptor, amplification products in parallel with MW standards are reported. Various tissues or cell lines were analysed in parallel as a positive controls: trigeminal ganglia for P2X2 and P2X3, total brain for P2X1, P2X4, P2X5, P2X7, P2Y13 and P2Y14, heart for P2X6, liver for P2Y4, lung for P2Y2 and N9 cells for P2Y1, P2Y6 and P2Y12. No products were detected in samples that did not undergo retrotranscription (–).