1.
Sequences of primers, hybridization probes and lengths of PCR products
| Gene | Size (bp) | Sequence 5'-3' | TAnnealing (°C) | |
|---|---|---|---|---|
| p53 | Real-time RT-PCR | 149 | Fw: ATGAGCCGCCTGAGGTTG Rev: AGCTGTTCCGTCCCAGTAGATTA FL: GGCATGAACCGGAGGCCCA-FlLC: Red640-CCTCACCATCATCACACTGGAAGACTCC-p | 62 |
| p21WAF1 | Real-time RT-PCR | 211 | Fw: GGCAGACCAGCATGACAGATT Rev: GCGGCCAGGGTATGTACATGA FL: CCTGTGGGCGGATTAGGGCTTCC-FlLC: Red640-CTTGGAGAAGATCAGCCGGCGTTT-p | 62 |
| β2-Microglobulin | Real-time RT-PCR | 269 | Fw: CCAGCAGAGAATGGAAAGTC Rev: GATGCTGCTTACATGTCTCG FL: TTCTTCAGTAAGTCAACTTCAATGTCGGA-FlLC: Red640-ATGAAACCCAGACACATAGCAATTCAG-p | 53 |
| p21WAF1 promoter (RE) | ChIP | 169 | Fw: AGGCTGTGGCTCTGATTGG Rev: GGCTAAGGTTTACCTGGGG | 60 |
| p21WAF1 promoter ChIP | ChIP | 150 | Fw: GGGCGGGGCGGTTGTATATCAG Rev: GTCTGCCGCCGCTCTCTCACCT | 60 |
Fw, forward; Rev, reverse; Fl, fluorescein labelled probe; LC, LCRed640 labelled probe; p, phosphorylated.