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. 2007 Aug 31;12(2):649–660. doi: 10.1111/j.1582-4934.2007.00112.x

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©2008 The Authors Journal compilation © 2008 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd

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Expression of VEGF in non-lesional psoriatic keratinocytes after calcium treatment. Non-lesional psoriatic keratinocytes from five cases of psoriasis were incubated with calcium at concentrations of 0, 0.5, 1.0, 2.0 and 3.0 mM. (A) mRNA expression of VEGFafter calcium treatment; GAPDH served as an internal control.(B) Scatter plots for RT-PCR band intensity ratio of VEGF gene versus GAPDH.(C) Western blot results of VEGF; GAPDH served as a loading control for protein normalization.(D) Scatter plot analysis of protein level of VEGF versus GAPDH as determined by Western blot.