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. 2007 Aug 31;12(2):649–660. doi: 10.1111/j.1582-4934.2007.00112.x

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©2008 The Authors Journal compilation © 2008 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd

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Effect of calcium and VEGF165 on expression of VEGFRs in non-lesional psoriatic keratinocytes.(A) Calcium regulates mRNA level of VEGFRs. Non-lesional psoriatic keratinocytes (n= 5) were incubated with calcium at concentrations of 0, 0.5, 1.0, 2.0 and 3.0 mM. GAPDH served as internal control for mRNA.(B) Relative quantitation of VEGFRs mRNA by real-time RT-PCR. The expression of VEGFRs was normalized to the endogenous control GAPDH. The relative fold change of VEGFRs in non-lesional psoriatic keratinocytes incubated with calcium was compared to that without calcium. The mRNA level of VEGFRs in NLPK treated with calcium at 1.0 mM is significantly higher than that in other indicated concentration (P <0.001). Bars: mean±SEM. (C) Bands at 180 KD and 200 KD are detected for VEGFR-1, VEGFR-2 and VEGFR-3. GAPDH served as a loading control.(D) Effect of VEGF165 and calcium with or without bevacizumab on protein levels of VEGFRs in non-lesional psoriatic keratinocytes. Non-lesional psoriatic keratinocytes (n= 3) were exposed to VEGF at 10ng/ml with or without 1.0mg/ml bevacizumab, and calcium at 1.0mM with or without 1.0mg/ml bevacizumab. Bands at 180 KD and 200 KD are detected for VEGFR-1, VEGFR-2 and VEGFR-3. GAPDH served as a loading control.