2.

8-epipuupehedione inhibits the production of MMP-2 (A) but not MMP-2 activity (B) and urokinase (C) and increases plasminogen activator inhibitor (PAI) levels (D) produced by HL-60 cells. Cells were treated for 24 hrs with 4 μM 8-epipuupehedione in serum-free culture medium containing aprotinin. After incubation, conditioned media were collected and concentrated 25x and cell extracts were obtained. For zymographic assays (carried out as described in the Material and methods section), conditioned media (for gelatinolytic asays) and cell extracts (for urokinase detection by plasminogen zymography) were normalized for equal cellular density.