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Substrates and products of LCAT. The principal reaction catalyzed by LCAT is transfer of an acyl group from the sn-2 position of phosphatidylcholine to the 3β-hydroxyl group of cholesterol. The broad sterol specificity of LCAT allows dehydroergosterol to be substituted for cholesterol in the assay and enable detection of dehydroergosteryl ester by fluorescence of its conjugated triene fluorophore.
