Fig. 1.

mRNA expressions of mechanosensitive ion channels determined by quantitative RT-PCR. Total RNA samples for liver, brain, thymus, heart, lung, spleen, testicle, ovary and kidney were obtained from 7–8 weeks old Sprague–Dawley rats. Total RNA sample for the embryo was obtained from midterm embryos of Sprague–Dawley rats. These RNA samples were purchased from Ambion. A total RNA sample for the rat ventricular cardiomyocyte cell line H9c2 was also obtained. TaqMan PCR primers were used for TRPA1, TRPC6, TRPM7, TRPV2, ASIC3 and TREK-1 channels' mRNAs. 18S ribosomal RNA was used as an internal control. Relative mRNA levels were calculated using ΔCt values (2∧(40 − ΔCt)) for each PCR run. Finally, the relative mRNA level was normalized to that of the embryo. All data are for three technical replicates.