fig 1.

Organization of the Nol3 gene and ARC sequence similarities. (A) Organization of the Nol3 locus. ARC is expressed from exons 2 to 4 of the Nol3 gene. Coding regions are shaded in grey. ATG and TGA indicate start and stop codons. Separating introns are shown as lines. (B) Alignment of mammalian ARC protein sequences. Mammalian ARC sequences were aligned using Clustal W software. Asterisks indicate identical amino acids, ‘:’ indicates conserved substitutions and ‘.’ indicates semi-conserved substitutions. The CARD region (aa 1–98) is highly conserved between all five species shown. The P/E rich region likewise presents with a high sequence similarity. (C) Domain organization of ARC. ARC comprises an NH₂-terminal CARD, followed by a region rich in proline and glutamate (P/E).