fig 6.

(A) Cytofluorimetry analysis of Cx43 expression. (B) Western Blot for dystrophin, Cx43 and cTnI; MW: molecular weight marker. Lane 1: PLLA/Ctrl seeded with C2C12 for 48 hrs; lane 2: PLLA/GCSF scaffold seeded with C2C12 for 48 hrs. A non-cardiac specific dystrophin antibody recognizing a 60 kD fragment of the native protein was used as a general marker for myoblasts. The contemporary presence of both myoblast and cardiac-specific markers could reliably suggest different stages in the differentiation process.