fig 2.

LTC-DC can cross-present antigen. LTC-DC and freshly isolated CD11c⁺ spleen cells (f-DC) from C57BL/6J mice were pulsed with OVA (10 μg/ml) ± LPS (10 μg/ml). Cells were co-cultured with purified CFSE-labelled CD8⁺ T cells from OT-I mice, or CD4⁺ T cells from OT-II mice. T cells alone served as a control (Nil). (A) Activation of CD8⁺ OT-I T cells was indicated by up-regulation of CD69 after 1 day, and by number of CD8⁺ T cells undergoing division based on CFSE dilution, or IFN-γ production after 4 days. (B) The number of CD4⁺ OT-II T cells undergoing division was assessed on the basis of CFSE dilution after 4 days.