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The CD45+ fraction of the peritoneal lavage cells obtained 48 hrs after injury of the peritoneal wall and purified by magnetic immunobeads, shows mesothelin mRNA expression by RT-PCR. Positive control (C+) consisted of mesothelial cells obtained by mild trypsinization of the peritoneal cavity. Negative control (C–) was performed with free peritoneal cells obtained from a mesothelin-null mouse. Normalization of RT-PCR product was made with expression of β-actin as reference gene.
