Fig. 6.

Effects of MAPK inhibition on chondrocyte redifferentiation. Chondrocytes collected after culture in the presence or absence of MAPK inhibitors were subjected to redifferentiation in pellet cultures containing osteo/chondrogenic differentiation medium without further addition of MAPK inhibitors. (A) Firm pellets were transferred to 6-well plates after 6 days of culture and an outgrowth assay was performed for another 6 days. Scale bar: 200 μm. (B) 10 μm cryosections of pellets were stained with Safranin O and Alcian blue for histological analysis of sulfated GAG content. Scale bar: 200 μm.