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. 2012 Jul 29;16(8):1776–1791. doi: 10.1111/j.1582-4934.2011.01473.x

Fig 3.

Fig 3

HSP27 expression patterns and heat-shock inducible HSP27 expression in pancreatic cancer cells. (A) Immunoblotting to assess constitutive HSP27 and phospho-HSP27 expression in 10 pancreatic cancer cell lines. β-ACTIN served as loading control. (B) Immunofluorescence to assess spatial HSP27 expression patterns in 5 pancreatic cancer cell lines. (C) Immunoblotting to assess inducible HSP27 expression upon heat shock (39°C and 41°C) in two established pancreatic cancer cell lines (PL5, PL11) and patient-derived short-term passaged primary pancreatic cancer cells (PPC-0039). β-ACTIN served as loading control.