Fig 9.

Effect of NS398, exogenous PGE₂ and combinational treatment of NS398 and exogenous PGE₂ on cell viability and VEGF, AQP1 mRNA transcription of HUVECs under normoxia or hypoxia. HUVECs were treated with 10 μM of NS398 and/or 10 μM of exogenous PGE₂ under normoxia or hypoxia for 3 hrs. Cells without NS398 or exogenous PGE₂ treatment served as control. (A) HUVECs proliferation and viability was measured by MTT assay. (B) VEGF mRNA level assessed by real-time RT-PCR experiment. (C) AQP1 mRNA level assessed by real-time RT-PCR experiment. *P < 0.05 and **P < 0.01 versus control.