Fig 3.

(A) ELISA quantification of BDNF expression in the DRG. BDNF expression in the lumbar DRG was quantified using ELISA. Rats induced to a state of EAE show significantly increased BDNF expression compared to NC animals (**P < 0.005; ***P < 0.001, ANOVA followed by Student’s t-test). Results are shown as pg/ml BDNF/μg total protein. (B) Real-time PCR results of BDNF expression within DRG. The BDNF mRNA expression of DRG for animals of the active EAE group (red bars), killed at day 12 is significantly higher than other groups (naïve control (blue bar) and active control (green bars)) (*P = 0.049) with a one way ANOVA and Tukey’s post test based on two factors (day and group) using software SPSS 16.0.