Fig 2.
p44 enhances AR and ER mediated transcriptional activation, p44 increased the levels of ER mediated transcriptional activation up to 2-fold (A) and increased the levels of AR mediated transcriptional activation up to 2.5-fold (B) in MCF7 cells in dual luciferase assays. In luciferase assays, the concentration of reagents where appropriate is as follow: androgen (10 nM R1881), oestrogen (10 nM 17-β-estradiol), AR (30 ng), ER (30 ng) and p44 (100 ng, 200 ng, 300 ng and 600 ng).