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. 2009 Oct 16;14(12):2780–2789. doi: 10.1111/j.1582-4934.2009.00936.x

Fig 4.

Fig 4

Nuclear p44 stimulates MCF7 breast cancer cell growth through oestrogen pathway, Growth curve of p44 showed increased MCF7 cell proliferation by NLSp44 compared with vector control (A) in oestrogen media. NLSp44 inhibited MCF7 cell growth in hormone free media (B) and NESp44 inhibited MCF7 cell growth in both hormone free (B) and oestrogen (A) media. In MDA-MB-231 cell line, the nuclear p44 inhibits and cytolasmic p44 promotes cancer cell growth both in oestrogen (C) and hormone free (D) media. Nuclear p44 mediated growth promotion is reversed by ERα knockdown in MCF7-NLSp44 cells compared to MCF7-NLSp44 treated with control siRNA (E) in oestrogen media. Cytoplasmic p44 expression in hormone free media (phenol red free and charcoal stripped FBS) (F, upper panel) and nuclear p44 expression in oestrogen media (F, lower panel). Increased ER target gene MYC expression by RT-PCR (G) and enhanced p44 recruitment to MYC promoter by ChIP (H) in the presence of oestrogen in MCF7-NLSp44 cells.