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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Biomol NMR Assign. 2012 Oct 16;7(2):10.1007/s12104-012-9431-9. doi: 10.1007/s12104-012-9431-9

Table 1. Diffusion Ordered Spectroscopy analysis of the Cx45CT in solution conditions that stabilize the dimer and monomer conformations.

Solution
Conditions		 Diffusion
Constant (m2/s)		 Hydrodynamic
Radius (Å)		 Calculated
Number of
Amino Acids		 Average
Molecular
Weight (kDa)		 Conformation
1× PBS 1.291E-10 25.1 313 34.41 Dimer
1× PBS with
30% acetonitrile		 1.275E-09 19.4 127 14.00 Monomer

Using the Stokes-Einstein equation, the hydrodynamic radius for each solution condition was calculated with the experimentally collected diffusion constant; D=KBT/(6ηπrH). The number of amino acids was then calculated based on the hydrodynamic radius (rH=4.75N^0.29), which was converted into an average molecular weight.