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. 2009 Dec 8;15(2):196–208. doi: 10.1111/j.1582-4934.2009.00989.x

Table 1.

Oligonucleotide sequences used for real-time PCR

Gene Sequences (5′ 3′) Size (bp) Hybridization T°C Cycles
TFPI-2 Forward: AACGCCAACAATTTCTACACCT Reverse: TACTTTTCTGTGGACCCCTCAC 109 67 35
MMP-1 Forward: CTGCTGCTGTTCTGGGGT Reverse: GCCACTATTTCTCCGCTTTTC 147 65 40
MMP-2 Forward: GGCCCTGTCACTCCTGAGAT Reverse: CAGTCCGCCAAATGAACCGG 105 67 34
MMP-3 Forward: ATCCCGAAGTGGAGGAAAAC Reverse: GCCTGGAGAATGTGAGTGGA 139 65 40
MMP-7 Forward: CCGCATATTACAGTGGATCG Reverse: GCCAATCATGATGTCAGCAG 111 60 40
MMP-9 Forward: AGACCGGTGAGCTGGATAG Reverse: GTGATGTTGTGGTGGTGCC 121 69 45
MMP-13 Forward: AGCATGGCGACTTCTACCC Reverse: CATCAAAATGGGCATCTCCT 96 65 40
MMP-14 Forward: CGAGGGGAGATGTTTGTCTT Reverse: TCGTAGGCAGTGTTGATGGA 131 65 40
EMMPRIN Forward: TGCTGGTCTGCAAGTCAGAG Reverse: GCGAGGAACTCACGAAGAAC 123 65 40
β-actine Forward: GCCCTAGACTTCGAGCAAGA Reverse: AGGAAGGAAGGCTGAAGAG 143 62 25