Table 1.
Oligonucleotide sequences used for real-time PCR
Gene | Sequences (5′ 3′) | Size (bp) | Hybridization T°C | Cycles |
---|---|---|---|---|
TFPI-2 | Forward: AACGCCAACAATTTCTACACCT Reverse: TACTTTTCTGTGGACCCCTCAC | 109 | 67 | 35 |
MMP-1 | Forward: CTGCTGCTGTTCTGGGGT Reverse: GCCACTATTTCTCCGCTTTTC | 147 | 65 | 40 |
MMP-2 | Forward: GGCCCTGTCACTCCTGAGAT Reverse: CAGTCCGCCAAATGAACCGG | 105 | 67 | 34 |
MMP-3 | Forward: ATCCCGAAGTGGAGGAAAAC Reverse: GCCTGGAGAATGTGAGTGGA | 139 | 65 | 40 |
MMP-7 | Forward: CCGCATATTACAGTGGATCG Reverse: GCCAATCATGATGTCAGCAG | 111 | 60 | 40 |
MMP-9 | Forward: AGACCGGTGAGCTGGATAG Reverse: GTGATGTTGTGGTGGTGCC | 121 | 69 | 45 |
MMP-13 | Forward: AGCATGGCGACTTCTACCC Reverse: CATCAAAATGGGCATCTCCT | 96 | 65 | 40 |
MMP-14 | Forward: CGAGGGGAGATGTTTGTCTT Reverse: TCGTAGGCAGTGTTGATGGA | 131 | 65 | 40 |
EMMPRIN | Forward: TGCTGGTCTGCAAGTCAGAG Reverse: GCGAGGAACTCACGAAGAAC | 123 | 65 | 40 |
β-actine | Forward: GCCCTAGACTTCGAGCAAGA Reverse: AGGAAGGAAGGCTGAAGAG | 143 | 62 | 25 |