Fig 7.

CD44⁺ CD24⁺ cells exhibit in vivo renal commitment when grafted under the renal capsule of contra-lateral kidney of unilateral nephrectomized mice. CD44⁺ CD24⁺ cells were grafted under the left kidney capsule of normal mice. One week after implantation, right kidney was nephrectomized. Grafts were harvested 2 weeks after nephrectomy. Photographs showing contra-lateral kidney of unilateral nephrectomized mice-control kidney (without graft) (A) and test kidney (with graft) (B). Haematoxylin and eosin stained section of the control kidney (without graft) (C) and test kidney (with graft) (D). Note the thick layer of cellular graft under the renal capsule in the test kidney which is absent in the control kidney. (Magnification = 100×). High magnification view of the graft sections showing neo-glomeruli like structures, vascularization (erythrocytes could be seen suggesting blood flow to the graft tissue) after 14 days of implantation (E). The dashed line separates the graft from the host. (Magnification = 200 ×). Grafts contained immature glomerular like structures (E.a, b), ducts (E.c, d) and vasculature with RBCs (E.e). The results are representative of five separate grafts. Abbreviations: CP, capillary; NG, neo-glomeruli; RBC, red blood cells