Fig 7.

Loss of β alters activation of signalling pathways in renal fibroblasts. Total and phosphorylated JNK, Erk1/Erk2 and mTOR were examined in homogenates from wild-type (A) and β^−/− (B) renal fibroblasts treated with TGF-β (1 ng/ml) for 15 min. by immunoblotting with specific antibodies. (C) Wild-type and β^−/− renal fibroblasts were cultured with high glucose (1.2 mM) for 48 hrs and then hypertrophy assessed by determination of protein : ratio. Total protein was measured by the Bradford method and DNA content was determined by propidium iodide fluorescence. Data shown are the mean ± S.E.M. of three independent experiments. **P< 0.01, two-way anova.