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. 2009 Oct 23;15(2):423–432. doi: 10.1111/j.1582-4934.2009.00948.x

Fig 3.

Fig 3

Fig 3

Ox-LDL suppressed endothelial differentiation of MAPCs. Endothelial differentiation of MAPCs was dose-dependently inhibited in the presence of ox-LDL with decreased expression of endothelial markers including vWF, Flk-1 and CD31, as well as impaired vascular structure formation. Native LDL had no impact on endothelial differentiation of MAPCs (data not shown). *P< 0.05 versus control (n= 4); **P< 0.01 versus control (n= 4). (A) The transcriptional expression of vWF (mRNA) in MAPCs during their endothelial differentiation was dramatically inhibited by ox-LDL as analysed by RT-PCR. (B) The majority of the cells stained positive for vWF after 2 weeks of endothelial differentiation under normal condition. (C) vWF protein content in the differentiating MAPCs was significantly decreased by ox-LDL as analysed by Western blot at week 2 of differentiation. (D) Ox-LDL substantially suppressed Flk-1 expression in the differentiating cells as analysed by Western blot at week 2 of differentiation. (E) CD31 expression in the differentiating cells was dramatically reduced by ox-LDL as analysed by Western blot at week 2 of differentiation.