Fig. 6.
Western Blot Analysis of Bcl-2, Phosphorylated Bad (pBad136) and Phosphorylated Akt (pAkt308) Expression in Mitochondrial and Cytosolic Fractions of Heart Tissue. Cytosolic (A) and mitochondrial (C) fractions of heart tissue were isolated at 24 hrs after burn, and subjected to Western blot analysis for Bcl-2, pBad136/Bad and pAkt308/Akt expression. GAPDH and ANT were used as loading controls for cytosolic and mitochondrial proteins respectively. The density of the Bcl-2, pBad136 and pAkt308 blot was, respectively, normalized against that of GAPDH (B), and the density of the pAkt308 blot was, respectively, normalized against that of ANT (D) to obtain relative blot density. **P < 0.01 versus Sham; ##P < 0.01 versus Burn.