Fig. 1.

(A) Progressive development of blood vessel-like structures in three dimensional fibrin-matrigel construct (in vitro) with different groups of cells (A-HUVECS, B-hCDSC and C-mix culture of HUVECS-hCDSC) on day 1, 3, 7 and 15. Images were captured using Inverted microscope with 100× magnification. (B) Three dimensional fibrin-matrigel construct with different groups of cells harvested from in vitro experiment. The vascular-like networks formed by HUVECS, hCDSC and mix culture of HUVECS-hCDSC were stained by Haematoxylin & Eosin. (C) Reactions control for immuno-staining. A-Negative control (without primary antibodies, ×200) and B-positive control (with primary antibodies, ×200) for PECAM-1, vWF and α-SMA. HUVECS monolayer culture was used for PECAM-1 and vWF reactions control (i, ii, iii, iv) whereas hCDSC monolayer culture were used for α-SMA reactions control (v, vi). (D) Three dimensional fibrin-matrigel construct with different groups of cells harvested from in vitro experiment. Immunostaining of PECAM-1, vWF and α-SMA were performed on vascular-like network formed by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) in the constructs in vitro. The arrow showed hematoxylin counterstained for the nucleus and the red scale bars represent the size of 30 μm.