Fig. 5.

(A) Three dimensional fibrin-matrigel construct with different groups of cells harvested from in vivo experiment. The vascular-like networks formed by HUVECS, hCDSC and mix culture of HUVECS-hCDSC were stained by Haematoxylin & Eosin. (B) Three dimensional fibrin-matrigel construct with different groups of cells harvested from in vivo experiment. Immunostaining of PECAM-1, vWF and α-SMA were performed on vascular-like network formed by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) in the three dimensional fibrin-matrigel construct in vivo. The red scale bars represent the size of 30 μm. (C) H&E staining of empty FMC harvested from in vivo experiment (A-i, ×100 and A-ii, ×200). The arrow showed infiltration of mouse cells. Mouse vessels stained with PECAM-1 antibody, with (B-iv, ×100) and without (B-iii, ×100) Rodent block M blocking reaction.