Fig 3.

Human skeletal muscle; laser scanning confocal microscopy; three-dimensional shadow projection image. Immunofluorescence labelling shows platelet-derived growth factor receptor (PDGFR)-β expression (red) in mural cells and a perivascular interstitial cell (arrowhead), especially along the long, thin cell prolongation (A). Same perivascular cell (arrowhead) is positive for c-kit (green); mural cells are marked by α-SMA (red) (B); co-localization for PDGFR-β and c-kit appears as yellow areas (arrowhead) (C). Nuclei are counterstained with 4′,6-diamidino-2-phenylindole (blue). Original magnification: 600×.