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qRT-PCR-primers and annealing temperatures
| Target | Forward primer | Reverse primer | Annealing temp (8C) |
|---|---|---|---|
| COL1A1 | TGCCATCAAATGCTTCTGC | CATACTCGAACTGGAATCCATC | 56 |
| COL3A1 | CCAGGAGCTAACGGTCTCAG | CAGGGTTTCCATCTCTTCCA | 48 |
| LOX | CCACTATGACCTGCTTGATG | TGTGGTAGCCATAGTCACAG | 60 |
| PLOD2 | AGCTGTGGTCCAATTTCTGG | CTAGCATTTCGGCAAAGAGC | 55 |
| GAPDH | ACAGTCAGCCGCATCTTC | GCCCAATACGACCAAATCC | 56 |
COL: collagen type 1 or 3; LOX: lysyl-oxidase; PLOD2: lysyl-hydroxylase; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
