Fig 3.

Pfn1 overexpression increased integrin β1 protein stability. (A) The expression level of integrin β1 mRNA determined by real-time PCR analysis; Agarose gel electrophoresis data showing relative level of integrin β1, Pfn1 and GAPDH (loading control) mRNA. (B) The stable transfected cells harvested at the indicated time after treatment with cycloheximide (100 μM) and the total protein extracts prepared for immunoblotting and GAPDH as a control; three independent experiments performed. (C, D) The cells treated with proteasome inhibitor MG132 (50 μM) or lysosome inhibitor chloroquine (20 μM), and the amount of integrin β1 assessed by Western blot.