Fig 6.

Actin superstructure maintenance was requisite of Pfn1-enhanced apoptosis under STS action. (A) Increased actin location in the plasma membrane determined by Western blot in Pfn1–468 compared to Mock cells; tubulin used as a control. (B) LatruculinB (4 μM) added before STS action and apoptosis measured by the estimation of cell viability with the CCK-8 assay; the units representing mean ± S.D. from three independent experiments; *differences significant at P < 0.05. (C) Equal amounts of total lysates analysed by immunoblot with antibodies against PARP and GAPDH; analysis of the band greyscales determined by Total Lab software shown in (D).