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Effect of allyl-isothiocyanate (AITC) and sulforaphane (SFN) on NF-κB nuclear translocation. Protein levels of p65, a subunit of the transcription factor NF-κB, were measured by Western blotting in nucleic extracts from RAW264.7 macrophages incubated with the test compounds (10 μmol/l) and stimulated with LPS (10 ng/ml) for six hrs. One representative Western blot is shown.
