Fig 7.

Effect of allyl-isothiocyanate (AITC) on Nrf2 and heme-oxygenase 1 (HO-1), in mice fed a pro-inflammatory high-fat diet. Female C57BL/6 mice were fed a pro-inflammatory high-fat diet for eight weeks. After seven weeks, the mice were applied 15 mg AITC per kg body weight or PBS (control) via oral gavage daily for seven days. Following RNA extraction from mouse liver levels of Nrf2 (A) and HO-1 (B) were measured by real-time PCR. Each bar represents the mean (±S.E.M.) from nine or 10 animals. Means without a common letter differ significantly (P < 0.05). Nuclear Nrf2 levels were determined in nucleic extracts derived from mouse liver via Western blotting (C). One representative Western blot with seven animals per group is shown.