3.

In vitro DNase I footprinting of 7/8 enhancer reveals two distinct protected regions. (A) A/G ladder used as reference (first lane); samples incubated with increasing amounts of Caco2 nuclear extracts (NE) and radiolabelled sense strand of 7/8 sequence. Lanes with NE alone and DNase I alone were used as controls. (B) Sequence corresponding to the two protected regions. Conservation track based on phastCons MultiZ alignment of 17 vertebrate species, available on ENCODE browser (http://www.genome.ucsc.edu/ENCODE). In silicoanalysis revealed a highly conserved HNF1 site on the antisense strand within FP1.