4.

Effect of Mrs2 ablation on mitochondrial membrane complexes. Clones MPL-O (with an empty vector), MPL-E1 and MPL-Q1 (expressing ptetHshE and ptetHshQ, respectively), marked here as WT, E and Q, were grown for 15 days in the presence of doxycycline. The mitochondria were isolated and proteins were solubilized in laurylmaltoside (1%). Respiratory chain complexes were resolved by BN-PAGE. One part (left) of the gel was stained with Coomassie Blue whereas the other part (right) was transferred onto nitrocellulose membrane and immunodeco-rated with sera against the 39 kD subunit of the complex I and against subunit IV of the complex IV.