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Down-regulation of Mrs2 expression decreases amounts of 39 kD subunit of complex I. HEK-293 clones MPL-O MPL-E1 or MPL-Q1 (designated as WT, E and Q) were grown for 15 days without doxycycline (non-induced) or for 5, 10 or 15 days in presence of doxycycline. Mitochondria were isolated and total mitochondrial proteins were separated by SDS-PAGE and immunoblotted using an antiserum against the 39 kD subunit of complex I (upper panel) or porin (lower panel).
