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APC stimulates type I collagen in tenocytes. (A) Tenocytes were incubated for 24 hrs in the presence or absence of APC. Cells were subjected to immuno-histochemical staining using an antibody to type I collagen. IgG was used as the isotype control. Bar = 20 μm. (B) Type I collagen in the cell supernatants in response to APC treatment for 24 hrs and detected by Western blot. β-actin was used as the loading control.
