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EPCR expression by cultured human tenocytes. Cells were treated with APC and incubated for 24 hrs. Expression of EPCR was detected by (A) Real time RT-PCR (graph) and PCR product was detected by 2% agarose gel with housekeeping gene GAPDH as an internal control (gel below graph) and (B) Western blot analysis, using cell lysates with β-actin used as the loading control and (C) immunohistochemical staining for EPCR in tenocytes (Bar = 20 μm).
