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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Cancer Discov. 2013 Aug 1;3(10):10.1158/2159-8290.CD-13-0118. doi: 10.1158/2159-8290.CD-13-0118

Figure 4. PLOD2 expression rescues sarcoma migration but not invasion.

Figure 4

(A) Quantification of migration assays of normoxic and hypoxic KIA cells expressing Scr or HIF1α specific shRNAs and ectopically expressing control or wild type human PLOD2 cDNA. All P values are ≤ 0.0432) (B) Quantification of HT-1080 migration assays performed as in (A) except murine Plod2 was ectopically expressed. All P values are ≤ 0.0431. (C) Representative images of boyden chamber migration assay using HT-1080 cells treated as in (B). (scale bar; 50 μm). (D) Quantification of invasion assays of normoxic and hypoxic KIA cells expressing Scr or HIF1α specific shRNAs and ectopically expressing control or wild type human PLOD2 cDNA using matrigel coated transwell invasion chambers. All P values are ≤ 0.0084. (E) Quantification of HT-1080 invasion assays performed as in (D) except murine Plod2 was ectopically expressed. All P values are ≤ 0.0017. (F) Representative images of matirgel coated chamber invasion assay using KIA cells treated as in (D) (scale bar; 50 μm).