Fig 1.

A subpopulation of ESC escapes neural differentiation. ESC were subjected to early neural differentiation by 5 days of coculture with PA6 stroma cells, then late differentiation was induced after cell dissociation and re-plating on polyornithine. (A) Flow cytometric analysis of nestin and βIII-tubulin expression during early differentiation. (B) Flow cytometric analysis of nestin and SSEA-1 expression during early differentiation. (C) Analysis of CFSE dilution by flow cytometry during early and late differentiation of ESC. (D) Combination of phenotypic and CFSE dilution analysis in differentiating ESC. The CFSE dilution was assessed at different time points for different subpopulations: nestin-positive/βIII-tubulin-negative (neuroepithelial cells), nestin-/ βIII-tubulin-positive (neuronal cells), nestin-/βIII-tubulin (non-neural cells).