Fig 2.

Variability of progenies derived from individual parental ESC. (A) In an experimental setup where one colony was derived from one parental ESC, cells were stained for nestin and βIII-tubulin after 72 hrs differentiation. (B) One hundred fifty ESC-derived colonies were analysed for the presence or not of NeuN (mature-stage neurons), TH (dopaminergic neurons) and βIII-tubulin (neuronal cells) cells. (C) ESC-Tα1-GFP were submitted to neural differentiation for 72 hrs and colonies were analysed for GFP expression. (D,E) ESC-H2B-mRFP1 were submitted to neural differentiation and monitored by live imaging during the first 2 days. The capacity of daughter cells to acquire a neural phenotype depended of the nature of parental ESC. Herein are presented three examples of different progenies.