Fig 4.

Clonal sublines do not share the neurogenic and cardiogenic potential. (A–C) Clonal ESC were submitted to neural differentiation by coculture on PA6 stromal cells. (A) The percentage of colonies including nestin-positive neuroepithelial cells was evaluated after 3 days. (B,C) The percentage of colonies including βIII-tubulin-positive neuronal cells (B) or (C) TH⁺ dopaminergic neurons was evaluated after 1 week. (D) ESC clones 1–4 were differentiated towards embryoid bodies. Cardiac differentiation was evaluated by the percentage of beating embryoid bodies at different time points. Statistical analysis was performed and all clonal ESC differed significantly from at least another one (Student’s t-test, P < 0.05).