Fig 6.

Clonal sublines from D3 confirms cellular diversity. (A) Total mRNA expression profile was performed on each clonal D3 subline. The expression of mRNA associated to pluripotency and/or early inner cell mass was established. (B) D3 sublines were submitted to neural differentiation by coculture on PA6 stromal cells. The percentage of colonies including βIII-tubulin-positive neuronal cells, NeuN⁺ mature neurons and TH⁺ dopaminergic neurons was evaluated after 1 week. Statistical analysis was performed and all clonal ESC differed significantly from at least another one (Student’s t-test, P < 0.05).