Fig 1.

TNF-α–induced overproduction of IL-8 in HCCs. (A) The levels of IL-8 in the supernatant harvested from HCCs challenged with PBS (None) or TNF-α at doses of 1, 5 and 10 ng/ml at 6 and 24 hrs. (B) The levels of IL-8 in the supernatant harvested from HCCs challenged with PBS or TNF-α at the dose of 1 ng/ml at 3, 6, 12, 18 and 24 hrs, respectively. Fifty ml of the supernatant was harvested and the same volume was added to measure the accumulative production of IL-8. (C) The levels of IL-8 in the supernatant harvested from HCCs challenged with PBS or TNF-α at the dose of 1 ng/ml during the period of 0–6, 6–12, 12–18 and 18–24 hrs. During every 6-hr period, the full supernatant was harvested and the same volume of fresh solution were added to evaluate every 6-hr production of IL-8.