Fig 4.

Effects of CCRs inhibitors on TNF-α–induced IL-8 production. (A) The levels of IL-8 were measured in the supernatant of HCCs pre-treated with CCR1, CCR2 and CCR3 inhibitors, UCB35625, RS504393 and SB328437, respectively, at the dose of 15.26 nM (10 ng/ml), 23.95 nM (10 ng/ml) and 26.424 nM (10 ng/ml) for 1 hr followed by the challenge with TNF-α at the dose of 1 ng/ml for 24 hrs. (B) The levels of IL-8 were measured in the supernatant of HCCs pre-treated with CCR3 inhibitor SB328437 at doses of 2.64 nM (1 ng/ml), 13.21 nM (5 ng/ml) and 26.42 nM (10 ng/ml) for 1 hr followed by the challenge with PBS or TNF-α at the dose of 1 ng/ml for 24 hrs.