Fig 5.

Effects of p38 inhibitor on TNF-α–induced activation of NF-κB. (A) The p65 activation (Optimal Density, OD) of NF-κB was measured in the nuclei of HCCs challenged with PBS or TNF-α at doses of 1, 5 and 10 ng/ml for 1 hr. (B) The p65 activation (OD) of NF-κB was measured in the nuclei of HCCs pre-treated with SB203580 at 5, 15 and 30 μM for 1 hr and challenged with PBS or TNF-α at the dose of 1 ng/ml for 1 hr. (C) Immunofluorescence staining of NF-κB p65 protein in HCCs pre-treated without or with SB203580 30 μM for 1 hr and challenged with PBS or TNF-α at the dose of 1 ng/ml for 1 hr.