Fig 7.

Bio-behaviours of hepatocellular cancer cells. Dynamic alterations in increased rate (%) of total cell number (A), stable cell number (B) and dead cell number (D) and decreased rate (%) of differentiated cell number (D) were measured by the real-time cell monitoring system, every 5 min. with 24 hrs after a 24 hrs culture of MHCC-97H cells pre-treated with vehicle (control), LY294002 (LY-5 μM), Wortmannin (WT-5 μM), SB203580 (SB-5 μM) and PD98059 (PD-5 μM) alone at the dose of 5 μM for 1 hr followed by the challenge with PBS or TNF-α at the dose of 1 ng/ml (TNF-α-1 ng/ml).