Fig 5.

Electrophysiological features of the neonatal cardiomyocytes cultured for 24 and 48 hrs in the absence (a) or presence of RLX (b). (A) Representative spontaneous action potentials recorded in current clamp mode at 24 hrs. (B) I_Na traces and (C) I_Ca traces recorded in voltage-clamp mode at 24 hrs: T indicates transient T-type I_Ca. (D) Boltzmann functions depicting I-V curves for I_Na peak, total I_Ca,tot and T-type Ca²⁺ currents, recorded in the presence of nifedipine at 24 and 48 hrs. The related Boltzmann parameters and the statistical analysis are reported in Table 2. Data are mean values from 26 to 35 different cells.